ATAD2 Antibody (CL0182) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14786
![Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Western-Blot-NBP2-14786-img0006.jpg "Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Immunohistochemistry-Paraffin-NBP2-14786-img0008.jpg "Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Immunohistochemistry-Paraffin-NBP2-14786-img0012.jpg "Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0182
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q6PL18, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected species cross reactivity based on sequence identity: Mouse( 82%), Rat (82%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ATAD2 Antibody (CL0182) - BSA Free
![Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Western-Blot-NBP2-14786-img0003.jpg "Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]")
Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]
Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Immunohistochemistry-Paraffin-NBP2-14786-img0009.jpg "Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Immunohistochemistry-Paraffin-NBP2-14786-img0010.jpg "Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Immunohistochemistry-Paraffin-NBP2-14786-img0011.jpg "Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Simple-Western-NBP2-14786-img0004.jpg "Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]")
![Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/ATAD2-Antibody-CL0182-Simple-Western-NBP2-14786-img0005.jpg "Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]")
![ATAD2 Antibody (CL0182) - BSA Free Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]](https://resources.bio-techne.com/images/products/nbp2-14786_-immunocytochemistry-immunofluorescence-639174076907389381.jpg "Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]")
Applications for ATAD2 Antibody (CL0182) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Simple Western
1:20
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:20, apparent MW was 182 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:20, apparent MW was 182 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATAD2
Long Name
ATPase family, AAA domain containing 2
Alternate Names
ANCCA, CT137, PRO2000
Gene Symbol
ATAD2
Additional ATAD2 Products
Product Documents for ATAD2 Antibody (CL0182) - BSA Free
Product Specific Notices for ATAD2 Antibody (CL0182) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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