Aspartate Transaminase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78089
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Aspartate Transaminase Antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart. (Uniprot: P00503)
Reactivity Notes
Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Aspartate Transaminase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000-1:25000
Western Blot
1:500-1:3000
Application Notes
This product is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Aspartate Transaminase
Alternate Names
aspartate aminotransferase, cytoplasmic, CASPAT
Gene Symbol
GOT1
UniProt
Additional Aspartate Transaminase Products
Product Documents for Aspartate Transaminase Antibody - BSA Free
Product Specific Notices for Aspartate Transaminase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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