Aquaporin-2 [p Ser261] Antibody - Azide Free

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100]

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).

Western Blot: Aquaporin-2 [p Ser261] Antibody - Azide Free [NB100-61100] -

(A) Expression of AQP2 in 1 week HU and REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against total AQP2. Densitometric analysis of total AQP2 bands normalized to the total protein content is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (*p < 0.05). (B) Expression of AQP2 in 1 week HU and REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against total AQP2. Densitometric analysis of total AQP2 bands normalized to the total protein content is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (**p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40303591), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Aquaporin-2 [p Ser261] Antibody - Azide Free [NB100-61100] -

(A) Expression of AQP2-pS261 in 4 weeks HU compared to REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against phosphorylated AQP2 in Ser261 and total AQP2. Densitometric analysis of AQP2-pS261 bands normalized to total AQP2 is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (*p < 0.05). (B) Localization of AQP2-pS261 and total AQP2. Immunofluorescence staining of AQP2-pS261, in green (AlexaFluor 488), and total AQP2, in red (AlexaFluor 555), in renal sections of HU and REST mice (scale bar: 5 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40303591), licensed under a CC-BY license. Not internally tested by Novus Biologicals.