AP2 alpha Antibody (24GB2485)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51563
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB2485
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human AP2 alpha (Uniprot: P05549)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AP2 alpha Antibody (24GB2485)
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (24GB2485)[NBP3-51563] -
Immunocytochemical staining of HepG2 cells with AP2 alpha antibody (1:1,000). Nuclei were stained blue with DAPI; AP2 alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: AP2 alpha Antibody (24GB2485)[NBP3-51563] -
Flow cytometric analysis of AP2 alphaexpression in HepG2 cells using AP2 alpha antibody (1:2,000). Green, isotype control; red, TFAP2A.
Western Blot: AP2 alpha Antibody (24GB2485)[NBP3-51563] -
Western blotting analysis using AP2 alpha antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for AP2 alpha Antibody (24GB2485)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AP2 alpha
Alternate Names
Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha
Gene Symbol
TFAP2A
Additional AP2 alpha Products
Product Documents for AP2 alpha Antibody (24GB2485)
Product Specific Notices for AP2 alpha Antibody (24GB2485)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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