Annexin A7 Antibody (JE65-36)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32039
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-36 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human Annexin A7 aa 1-250/488. (Uniprot: P20073)
Localization
Endoplasmic reticulum membrane, extracellular exosome, nucleus, collagen-containing extracellular matrix, cytoplasm, membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Annexin A7 Antibody (JE65-36) (NBP3-32039) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Annexin A7 Antibody (JE65-36)
Western Blot: Annexin A7 Antibody (JE65-36) [NBP3-32039]
Western Blot: Annexin A7 Antibody (JE65-36) [NBP3-32039] - Western blot analysis of Annexin A7 on SH-SY5Y cell lysates with Rabbit anti-Annexin A7 antibody (NBP3-32039) at 1/500 dilution.Lysates/proteins at 10 ug/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32039) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for Annexin A7 Antibody (JE65-36)
Application
Recommended Usage
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Annexin A7
Annexins are a multigene family of Ca++-regulated phospholipid-binding proteins. They serve as membrane-membrane, or membrane-cytoskeleton linkers, and regulate the formation of lipid domains in the plasma membrane. The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments.
Alternate Names
ANX7, ANXA7, Synexin
Gene Symbol
ANXA7
Additional Annexin A7 Products
Product Documents for Annexin A7 Antibody (JE65-36)
Product Specific Notices for Annexin A7 Antibody (JE65-36)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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