AMPK beta 1 Antibody (23GB2315)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50255

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2315

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK beta 1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human AMPK beta 1 (Uniprot: Q9Y478)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK beta 1 Antibody (23GB2315)

AMPK beta 1 Antibody (23GB2315)

Immunocytochemistry/ Immunofluorescence: AMPK beta 1 Antibody (23GB2315)[NBP3-50255] -

Immunocytochemical staining of H9C2 cells with AMPK beta 1 antibody (1:1,000) . Nuclei were stained blue with DAPI; AMPK beta 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.

Applications for AMPK beta 1 Antibody (23GB2315)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK beta 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).

Long Name

AMP-activated Protein Kinase beta 1

Alternate Names

PRKAB1

Gene Symbol

PRKAB1

Additional AMPK beta 1 Products

Product Documents for AMPK beta 1 Antibody (23GB2315)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK beta 1 Antibody (23GB2315)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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