Alpha-parvin/Actopaxin Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00222
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human Alpha-parvin/Actopaxin (Uniprot#: Q9NVD7-1; Met1-Glu372).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Alpha-parvin/Actopaxin Antibody - Azide and BSA Free
Western Blot: Alpha-parvin/Actopaxin Antibody [NBP3-00222] -
Western Blot: Alpha-parvin/Actopaxin Antibody [NBP3-00222] - NBP3-00222 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:48 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Alpha-parvin/Actopaxin Antibody [NBP3-00222] -
Immunoprecipitation: Alpha-parvin/Actopaxin Antibody [NBP3-00222] - PARVA was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00222 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00222, at 1:200 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDaApplications for Alpha-parvin/Actopaxin Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha-Parvin
Alternate Names
Actopaxin, alphaParvin, CH-ILKBP, MXRA2, PARVA
Gene Symbol
PARVA
Additional alpha-Parvin Products
Product Documents for Alpha-parvin/Actopaxin Antibody - Azide and BSA Free
Product Specific Notices for Alpha-parvin/Actopaxin Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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