Alpha Amylase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77792
![Western Blot: Alpha Amylase Antibody [NBP1-77792]](https://resources.bio-techne.com/images/products/Alpha Amylase Antibody-Western Blot-NBP1-77792-img0002.jpg "Western Blot: Alpha Amylase Antibody [NBP1-77792]")
Key Product Details
Species Reactivity
Bacteria
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Alpha Amylase [Bacillus amyloliquefaciens] (Uniprot: P00692)
Reactivity Notes
Reactive with Alpha Amylase from Bacillus amyloliquefaciens.
Cross reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined
Cross reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Alpha Amylase Antibody - BSA Free
Western Blot: Alpha Amylase Antibody [NBP1-77792]
Western Blot: Alpha Amylase Antibody [NBP1-77792] - Analysis using the Biotin conjugate of NBP1-77792. Detection of Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha Amylase antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin.Applications for Alpha Amylase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by ELISA and western blot and is assayed against 1.0 ug of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Alpha Amylase
Alternate Names
AMY2;AMY2A;AMY2B;AMY3;HXA;PA
UniProt
Additional Alpha Amylase Products
Product Documents for Alpha Amylase Antibody - BSA Free
Product Specific Notices for Alpha Amylase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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