Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09106
Key Product Details
Species Reactivity
Human, Porcine, Canine, Goat, Monkey, Sheep
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # W8B2B10
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
WERI-RB-1 retinoblastoma cell line
Specificity
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free (NBP3-09106) is a monoclonal antibody validated for use in Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images
Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] -
Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).
Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] -
Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Anti-Hu TNAP purified antibody (concentration in sample 0.3 ug/ml, GAM APC, red-filled histogram) binds specifically to TNAP on subpopulation of HeLa cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).Applications
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Tissue Non-Specific
Alternate Names
Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935
Gene Symbol
ALPL
Additional Alkaline Phosphatase, Tissue Non-Specific Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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