AKAP13 Antibody

Western Blot: AKAP13 Antibody [NB100-68214]

Western Blot: AKAP13 Antibody [NB100-68214] - Detection of Human AKAP13/AKAP-Lbc on HeLa whole cell lysate using NB100-68214. AKAP13/AKAP-Lbc was also immunoprecipitated by rabbit anti-AKAP13/AKAP-Lbc antibody NB100-68213.

Western Blot: AKAP13 Antibody [NB100-68214] -

Characterisation of LNX1 interacting proteins.(A) Schematic diagram of the domain structure of LNX1p80 and LNX2 showing the RING and four PDZ domains. N represents the NUMB-binding NPAY/NPAF motif. (B) The ability of the indicated proteins to interact with transfected GFP-tagged LNX constructs was assessed in HEK 293 cells. For each interacting protein, top panels show western blots of cell lysates (Lys), while the bottom panels show the output of a GFP “pull down" assay (PD). In the panels on the left, the specificity of interactions for LNX1 versus LNX2 was assessed, while on the right the interaction site on LNX1 was mapped to individual protein domains. Binding of endogenous proteins to LNX was assessed for liprin alpha-1, KIF7 and NUMB. For the other proteins, interactions of transfected HA or GST epitope-tagged proteins were assessed. For AKAP13, the mapping to LNX domains was performed in two separate experiments. Successful expression and pull down of GFP-tagged LNX proteins was verified in all assays and representative “pull down" blots probed for GFP are shown. n = 2–3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29121065), licensed under a CC-BY license. Not internally tested by Novus Biologicals.