ADNP Antibody - BSA Free

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Western Blot: ADNP Antibody [NBP1-89236]

Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot Shows ADNP Specificity Using Knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP1-89236) followed by HRP-conjugated secondary antibody. A specific band was detected for ADNP at approximately 135 kDa (as indicated) in the parental DMS 53 cell line, but is significantly reduced in knockdown DMS 53 cell line. Primary antibody concentration used: 0.4 μg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of ADNP by Immunoprecipitation.

DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP1-89236) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot with a Rabbit ADNP antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).