ADAR Antibody (CL0176) - BSA Free

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human cerebral cortex shows nuclear positivity.

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human testis shows nuclear positivity.

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human kidney shows strong nuclear positivity in cells in tubules.

Western Blot: ADAR Antibody (CL0176) - BSA Free [NBP3-05500] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.