ACOT8 Antibody (3F1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00010005-M03

Novus Biologicals, part of Bio-Techne
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H00010005-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F1

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACOT8 Antibodies »

Product Specifications

Immunogen

ACOT8 (NP_005460.2, 108 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYI

Specificity

Reacts with acyl-CoA thioesterase 8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse ACOT8 Antibody (3F1) - Azide and BSA Free (H00010005-M03) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ACOT8 Antibody (3F1) - Azide and BSA Free

Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]

Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]

Western Blot: ACOT8 Antibody (3F1) [H00010005-M03] - Analysis of ACOT8 expression in A-431 (Cat # L015V1).
Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]

Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]

Western Blot: ACOT8 Antibody (3F1) [H00010005-M03] - Analysis of ACOT8 expression in transfected 293T cell line by ACOT8 monoclonal antibody (M03), clone 3F1. Lane 1: ACOT8 transfected lysate (Predicted MW: 35.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: ACOT8 Antibody (3F1) [H00010005-M03] - Detection limit for recombinant GST tagged ACOT8 is 1 ng/ml as a capture antibody.

Applications for ACOT8 Antibody (3F1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACOT8

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACTEIII, acyl-CoA thioesterase 8HNAACTE, Choloyl-coenzyme A thioesterase, EC 3.1.2.27, hACTEIII, HIV-Nef-associated acyl-CoA thioesterase, hTEcholoyl-CoA hydrolase, long-chain fatty-acyl-CoA hydrolase, palmitoyl-CoA hydrolase, peroxisomal acyl-CoA thioesterase, peroxisomal acyl-CoA thioesterase 1, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, PTE-1, PTE1HIV-Nef associated acyl-CoA thioesterase, PTE2, PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8, Thioesterase II, thioesterase III

Entrez Gene IDs

10005 (Human)

Gene Symbol

ACOT8

UniProt

NP_005460 (Human)

Additional ACOT8 Products

Product Documents for ACOT8 Antibody (3F1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACOT8 Antibody (3F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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