ACE-2 Antibody (MSVA-919R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52492
Recombinant Monoclonal Antibody
![ACE-2 Antibody (MSVA-919R) Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]](https://resources.bio-techne.com/images/products/nbp3-52492_-immunohistochemistry-639196686453922097.jpeg "Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-919R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary)
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
Positive Control
Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane.
Negative Control
Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining.
Scientific Data Images for ACE-2 Antibody (MSVA-919R) - Azide and BSA Free
Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]
Leydig cells and spermatocytes of the testis show a particularly strong ACE2 immunostaining in the testis.![ACE-2 Antibody (MSVA-919R) Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]](https://resources.bio-techne.com/images/products/nbp3-52492_-immunohistochemistry-639196686509134829.jpeg "Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]")
Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]
A strong ACE2 immunostaining occurs in proximal tubuli of the kidney.![ACE-2 Antibody (MSVA-919R) Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]](https://resources.bio-techne.com/images/products/nbp3-52492_-immunohistochemistry-639196686633107496.png "Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]")
Immunohistochemistry: ACE-2 Antibody (MSVA-919R) - Azide and BSA Free [NBP3-52492]
Clear cell renal cell carcinoma strong membranous ACE2 positivity.
Applications for ACE-2 Antibody (MSVA-919R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ACE-2
Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.
Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).
References
1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., . . . Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145
2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7
3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S., . . . Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052
Long Name
Angiotensin I Converting Enzyme 2
Alternate Names
ACE2, ACEH
Gene Symbol
Q9BYF1, ACE2
Additional ACE-2 Products
Product Documents for ACE-2 Antibody (MSVA-919R) - Azide and BSA Free
Product Specific Notices for ACE-2 Antibody (MSVA-919R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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