ACAA1 Antibody (CL2662) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42381
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0002.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Western-Blot-NBP2-42381-img0001.jpg "Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL2662
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P09110, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected to cross react based on sequence identity: Mouse (88%), Rat (87%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ACAA1 Antibody (CL2662) - BSA Free
Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]
Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251![Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunocytochemistry-Immunofluorescence-NBP2-42381-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]")
Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]
Immunocytochemistry/Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381] - Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody, showing specific staining in peroxisomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0006.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]
Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0003.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0004.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0005.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
Applications for ACAA1 Antibody (CL2662) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ACAA1
Alternate Names
Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 1, EC 2.3.1, peroxisomal
Gene Symbol
ACAA1
UniProt
Additional ACAA1 Products
Product Documents for ACAA1 Antibody (CL2662) - BSA Free
Product Specific Notices for ACAA1 Antibody (CL2662) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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