6 Phosphofructo 2 Kinase Antibody (7X10D9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16295
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7X10D9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human 6 Phosphofructo 2 Kinase (NP_004557.1). LDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit 6 Phosphofructo 2 Kinase Antibody (7X10D9) (NBP3-16295) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for 6 Phosphofructo 2 Kinase Antibody (7X10D9)
![Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]](https://resources.bio-techne.com/images/products/6-Phosphofructo-2-Kinase-Antibody-7X2H3-Western-Blot-NBP3-16295-img0004.jpg "Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]")
Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]
Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X2H3) [NBP3-16295] - Western blot analysis of extracts of various cell lines, using 6 Phosphofructo 2 Kinase Rabbit mAb (NBP3-16295) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295] -
Immunohistochemistry: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 6 Phosphofructo 2 Kinase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295] -
Immunohistochemistry: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using 6 Phosphofructo 2 Kinase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.Applications for 6 Phosphofructo 2 Kinase Antibody (7X10D9)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 -1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PFKFB3
Long Name
6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase
Alternate Names
6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2
Gene Symbol
PFKFB3
Additional PFKFB3 Products
Product Documents for 6 Phosphofructo 2 Kinase Antibody (7X10D9)
Product Specific Notices for 6 Phosphofructo 2 Kinase Antibody (7X10D9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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