4EBP1 [p Thr46] Antibody (24GB4405)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51640

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4405

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 4EBP1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-4EBP1 (Thr46) (Uniprot: Q13541)

Modification

p Thr46

Specificity

This antibody detects endogenous levels of EIF4EBP1 only when phosphorylated at T46

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for 4EBP1 [p Thr46] Antibody (24GB4405)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 4EBP1

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

Additional 4EBP1 Products

Product Documents for 4EBP1 [p Thr46] Antibody (24GB4405)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 4EBP1 [p Thr46] Antibody (24GB4405)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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