14-3-3 gamma Antibody (23GB1970)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50963
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1970
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human 14-3-3 gamma (Uniprot: P61981)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 14-3-3 gamma Antibody (23GB1970)
Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma Antibody (23GB1970)[NBP3-50963] -
Immunocytochemical staining of HepG2 cells with 14-3-3 gamma antibody (1:1,000). Nuclei were stained blue with DAPI; 14-3-3 gamma was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: 14-3-3 gamma Antibody (23GB1970)[NBP3-50963] -
Validation of 14-3-3 gamma knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with 14-3-3 gamma antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: 14-3-3 gamma Antibody (23GB1970)[NBP3-50963] -
Flow cytometric analysis of 14-3-3 gammaexpression in HepG2 cells using 14-3-3 gamma antibody (1:2,000). Green, isotype control; red, 14-3-3 gamma.Applications for 14-3-3 gamma Antibody (23GB1970)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 14-3-3 gamma
Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.
14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.
Alternate Names
1433 gamma, YWHAG
Gene Symbol
YWHAG
Additional 14-3-3 gamma Products
Product Documents for 14-3-3 gamma Antibody (23GB1970)
Product Specific Notices for 14-3-3 gamma Antibody (23GB1970)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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