Does this kit contain the full-length or truncated form of PARP?

This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.

What is the maximum DMSO concentration in samples that can interfere with the assay?

Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.

What wavelength should be used to read the chemiluminescent plate?

The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.

Universal Chemiluminescent PARP Assay - Histone Coated Wells

Name: Universal Chemiluminescent PARP Assay - Histone Coated Wells
Brand: R&D Systems
SKU: 4676-096-K

R&D Systems, part of Bio-Techne | Catalog # 4676-096-K

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Product Datasheet / COA

For the in vitro screening of candidate PARP-1 inhibitors

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Product Summary
Kit Contents
Preparation & Storage
Background
Product Documents

Key Product Details

Features

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

Chemiluminescent readout
96 strip-well format
Sensitive – detects 10 mU PARP /well
Assay Time ~3 hrs

Species

Multi-Species

Product Summary for Universal Chemiluminescent PARP Assay - Histone Coated Wells

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents for Universal Chemiluminescent PARP Assay - Histone Coated Wells

3-Aminobenzamide
Human PARP 1 Enzyme, HSA
20X PARP Buffer
10X PARP Cocktail
10X Activated DNA
10X Strep-Diluent
PeroxyGlow™ A
PeroxyGlow™ B
Histone-Coated White Strip Well Plate
Strep-HRP

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PARP

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Additional PARP Products

Product Documents for Universal Chemiluminescent PARP Assay - Histone Coated Wells

Product Datasheets

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