Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

Name: Sialyltransferase Activity Kit
Brand: R&D Systems
SKU: EA002

The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

Sialyltransferase Activity Kit

R&D Systems, part of Bio-Techne | Catalog # EA002

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Citations (10)

Product Datasheet / COA / SDS

Measurement of sialyltransferase activity

Summary for Sialyltransferase Activity Kit

The Sialyltransferase Activity Kit (Catalog # EA002) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sialyltransferases regardless of different acceptor substrates. This kit utilizes the 5’-nucleotidase CD73 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide cytidine 5’-monophosphate (CMP) that is generated during sialyltransferase reactions.

Features

Applicable for all sialyltransferases
Non-radioactive
No time-consuming separation steps (e.g. column chromatography)
Provides accurate kinetic analysis of sialyltransferase reactions
Amenable to high-throughput analysis

Kit Contents

CHO cell-expressed Recombinant Human CD73
CMP
Phosphatase Buffer 2
100 mM MnCl₂
Phosphate Standard
Malachite Green Reagents A & B

Data Example

Activity of ST6GALNAC2

The ability of Recombinant Mouse ST6 Sialyltransferase 2/ST6GALNAC2 (Catalog # 6468-GT) to transfer Neu5Ac (sialic acid) from CMP-Neu5Ac to bovine asialofetuin was measured using the Sialyltransferase Activity Kit (Catalog # EA002). The activity was plotted against enzyme input, and the specific activity of ST6GALNAC2 was determined to be 774 pmol/min/μg.

Product Specifications for Sialyltransferase Activity Kit

Species	Multi-Species
Source	N/A

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Sialyltransferase Activity Kit

R&D Systems, part of Bio-Techne | Catalog # EA002

Key Product Details

Summary for Sialyltransferase Activity Kit

Features

Kit Contents

Data Example

Activity of ST6GALNAC2

Product Specifications for Sialyltransferase Activity Kit

Preparation & Storage

Customer Reviews for Sialyltransferase Activity Kit

FAQs for Sialyltransferase Activity Kit

Product Documents for Sialyltransferase Activity Kit

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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