jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # KA1526
Key Product Details
Sample Type & Volume Required Per Well
Nuclear extracts or JMJD2 subtypes (JMJD2A-JMJD2F) using purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues (1- 4 uL)
Sensitivity
1 ug nuclear extract; 10 ng purified enzyme
Assay Range
1 to 20 ug nuclear extract; 10 to 500 ng purified enzyme
Product Specifications
Assay Type
Colorimetric
Kit Type
Activity/Inhibition Assay Kit (Colorimetric)
Description
Quality control test: Standard Curve JMJD2 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening JMJD2 demethylase inhibitors.
Scientific Data Images for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)
jmjd-2 Assay/Inhibitor Screening Assay Kit [KA1526] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Kit Contents for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)
- 8-Well Assay Strips (With Frame)
- Adhesive Covering Film
- Co-factor 1
- Co-factor 2
- Co-factor 3
- JA1 (10X Wash Buffer)
- JA2 (JMJD2 Assay Buffer)
- JA3 (JMJD2 Substrate, 50 µg/mL)
- JA4 (JMJD2 Assay Standard, 50 µg/mL)
- JA5 (Capture Antibody, 1000 µg/mL)
- JA6 (Detection Antibody, 400 µg/mL)
- JA7 (Developer Solution)
- JA8 (Stop Solution)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: jmjd-2
Alternate Names
JMJD2
Additional jmjd-2 Products
Product Documents for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)
Product Specific Notices for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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