High Throughput Superoxide Dismutase Assay Kit

In the HT Superoxide Dismutase Assay Kit, superoxide radical (O2 –) ions, generated from the conversion of xanthine to uric acid and H2 O2 by xanthine oxidase (XOD), convert WST-1 to WST-1 Formazan. WST-1 Formazan absorbs light at 450 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of WST-1 formazan formation. The extent of reduction in the appearance of WST-1 Formazan is a measure of SOD activity present in your experimental sample. Superoxide dismutases (SODs) catalyze the dismutation of the superoxide radical (O2 –) into hydrogen peroxide (H2 O2). The HT Superoxide Dismutase Assay Kit is free of interference by other catalytic activities and is ideal for assaying SOD in mammalian tissue and cell lysates in a 96 well format. Unlike some other assay kits for SOD, this system is minimally disturbed by trace metals. Sufficient reagents are provided for 480 tests. The assay is performed in as little as 6 minutes and relative SOD activity of the experimental sample is determined as the percent inhibition of the rate of formation of WST-1 Formazan. This assay is also suitable for the assay of isozymes SOD1 (cytosolic Cu/Zn-SOD), SOD2 (mitochondrial Mn-SOD), and SOD3 (extracellular Cu/Zn-SOD).