How long can CometSlides™ be stored after electrophoresis?

Prior to staining, slides immersed in 70% ethanol for 5 minutes and then dried can be stored at room temperature for 1 year prior to staining. Permanent records can be created after staining for visualization by standard light microscopy using the CometAssay® Silver Staining Kit (Catalog # 4251-050-K).

What can cause observation of artefacts during imaging of Comet data?

For optimal imaging, all cells should be in the same plane and this is achieved by completely drying the agarose. Artefacts may be introduced if the source cells used for analyis has any debris. The debris should be allowed to settle before pipetting cells. The LMagarose can also be the cause of artefacts. To prevent debris from accumulating in the agar, melt it at 80-90 °C so it is runny, spin it at low speed and aliquot into sterile tubes in a hood. Use each aliquot for a single assay.

CometAssay Single Cell Gel Electrophoresis Assay-Silver

Name: CometAssay Single Cell Gel Electrophoresis Assay-Silver
Brand: R&D Systems
SKU: 4251-050-K

R&D Systems, part of Bio-Techne | Catalog # 4251-050-K

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Product Datasheet / COA

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Product Summary
Kit Contents
Preparation & Storage
Product Documents

Key Product Details

Features

Includes CometAssay reagents and components for the convenient silver staining of single cell gel electrophoresis samples.

Key Benefits

Allows visualization by standard light microscopy
Permanent staining for archiving samples
Optimized for CometSlide staining

Product Summary for CometAssay Single Cell Gel Electrophoresis Assay-Silver

Designed specifically for use with the CometSlides to minimize unwanted background and the amount of hazardous waste generated by silver nitrate. The silver staining components may be purchased separately.

Product Specifications

CometAssay and FLARE

NOTE: Once reconstituted 2X Staining Reagent #4 is stable for 3 months at 4°C.

Kit Contents for CometAssay Single Cell Gel Electrophoresis Assay-Silver

CometAssay Lysis Solution
CometAssay LMAgarose
CometSlides (2 Well, 25 Slides)
200 mM EDTA, pH 10
20X Staining Reagent #1
20X Staining Reagent #2
20X Staining Reagent #3
2X Staining Reagent #4
10X Fixation Additive< /br>Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.

Storage

Store the unopened product at room temperature. Do not use past expiration date.

CometAssay Single Cell Gel Electrophoresis Assay-Silver

R&D Systems, part of Bio-Techne | Catalog # 4251-050-K

Key Product Details

Features

Key Benefits

Product Summary for CometAssay Single Cell Gel Electrophoresis Assay-Silver

Kit Contents for CometAssay Single Cell Gel Electrophoresis Assay-Silver

Formulation, Preparation, and Storage

Shipping

Storage

Product Documents for CometAssay Single Cell Gel Electrophoresis Assay-Silver

Product Datasheets

COA

Certificate of Analysis

CONTACT INFORMATION

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