CardioTACS In Situ Apoptosis Detection Kit

The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system. R&D Systems has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label in apoptotic cells.