Multi-omic spatial analysis with simultaneous detection of small RNAs, mRNAs and proteins using the novel RNAscope™ Plus technology
by A Dikshit, S Basak, S Deshpande, M Sambhi, L-C Wang and M Srinivasan. Advanced Cell Diagnostics, a Bio-Techne brand
Scientific Meeting PostersScientific Meeting Posters Summary
The RNAscope™ Plus assay can detect 1 small RNA and 3 mRNA targets using TSA-based fluorescent readouts and is compatible with the new fluorescent TSA Vivid™ dyes. Fixed, fresh frozen samples and formalin fixed paraffin embedded (FFPE) tissues are supported by manual and automated workflows using the Leica Bond Rx system. We have leveraged this technology to investigate spatial expression profile of miRNA and associated RNA targets across different tissue types and applications. We can also combine protein detection using a target antibody to visualize cell-type specific markers.
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