This application note highlights how Simple Plex™ Assays measure interferon-gamma (IFN-γ) secretion as a direct readout of CAR-T cell activity, delivering a streamlined, quantitative approach to potency testing.
Why potency testing matters for CAR-T programs
As CAR-T programs scale, assays must be fast, precise, reproducible, and meet regulatory expectations. Reliable potency data support product consistency and release decisions.
How Simple Plex Assays evaluate potency
Using co-culture models with antigen-positive and -negative targets, Simple Plex IFN-γ Assays provide:
- High sensitivity and strong specificity
- Clear antigen-driven response discrimination
- Alignment with orthogonal cytotoxicity assays
Fast, Automated, and Reproducible
- Fully automated cartridges reduce manual steps and variability
- Quantitative results in ~90 minutes on a 21 CFR Part 11–compliant Ella™ system
- A scalable, GMP-ready solution for robust CAR-T potency assessment.
