Summary
The immune system plays a critical role in combating several types of malignancies. Immunotherapies including checkpoint blockade therapies have utilized the innate ability of the immune system to identify and eliminate malignant cells. It has also been established that the profile of tumor-infiltrating lymphocytes (TILs) can to some extent predict patient response and overall survival. Spatial technologies that enable the simultaneous detection of cytokines and chemokines with immune cell markers, can provide information about the immune cell composition and enable a comprehensive understanding of mechanisms underlying immune recruitment, infiltration and exclusion.
The use of RNAscope™ HiPlex Pro on COMET along with seqIF allows true multiomic analysis with simultaneous visualization of RNA and protein targets for immune cell profiling. Detecting cytokine and chemokine expression provides vital information about immune cell activation and recruitment, thereby increasing our understanding of phenomenon such as immune exclusion which is key in establishing predictive signatures for immunotherapy response.
This poster was presented at the AACR 2025 meeting.
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