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Using Single-Cell Westerns to Validate Single-Cell RNA-Seq Data

Single-Cell Westerns offer the versatility to validate diverse protein targets discovered in your sequencing runs

Single-cell technologies allow researchers to identify rare cell subpopulations that play a key role in disease and profile variation in patient treatment responses. RNA-Seq measures transcript levels in thousands of individual cells to determine RNA expression heterogeneity. However, mRNA transcript levels do not always correlate with levels of functional protein due to translational and post-translational regulation, micro-RNA inhibition, or protein degradation. Watch this video to learn how you can use the Milo™ Single-Cell Western platform, in parallel with Single-Cell RNA-Seq, to validate RNA data with protein levels in a heterogeneous cell population.