TRIS base
Tocris Bioscience, part of Bio-Techne | Catalog # 3163
Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Key Product Details
Description
Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Product Description
TRIS base is a commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS), Tris-acetate-EDTA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.Product Specifications
Molecular Weight
121.14
Formula
C4H11NO3
Storage
Store at RT
Purity
≥99.5%
Chemical Name
2-Amino-2-(hydroxymethyl)-1,3-propanediol
CAS Number
77-86-1
PubChem ID
6503
InChI Key
LENZDBCJOHFCAS-UHFFFAOYSA-N
SMILES
OCC(CO)(N)CO
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 363.42 | 3000 |
Preparing Stock Solutions
for TRIS base
The following data is based on the product molecular weight 121.14
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 30 mM | 0.28 mL | 1.38 mL | 2.75 mL |
| 150 mM | 0.06 mL | 0.28 mL | 0.55 mL |
| 300 mM | 0.03 mL | 0.14 mL | 0.28 mL |
| 1500 mM | 0.01 mL | 0.03 mL | 0.06 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for TRIS base
For research use only
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