Sulfo-NHS-LC-Biotin

Name: Sulfo-NHS-LC-Biotin
Brand: Tocris Bioscience
SKU: 7302

Tocris Bioscience, part of Bio-Techne | Catalog # 7302

Citations (3)

COA / SDS

Amine reactive biotinylation reagent, cell impermeable

Key Product Details

Description:	Amine reactive biotinylation reagent, cell impermeable
Chemical Name:	Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate
Molecular Weight:	556.58

View Full Technical Details for Sulfo-NHS-LC-Biotin

Technical Data
Citations

Biological Activity for Sulfo-NHS-LC-Biotin

Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Technical Data for Sulfo-NHS-LC-Biotin

M. Wt	556.58
Formula	C₂₀H₂₉N₄NaO₉S₂
Storage	Store at -20°C
CAS Number	191671-46-2
PubChem ID	71571497
InChi Key	JJGWLCLUQNFDIS-GTSONSFRSA-M
Smiles	[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Sulfo-NHS-LC-Biotin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	55.66	100
	DMSO	55.66	100

Preparing Stock Solutions for Sulfo-NHS-LC-Biotin

The following data is based on the product molecular weight 556.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.80 mL	8.98 mL	17.97 mL
5 mM	0.36 mL	1.80 mL	3.59 mL
10 mM	0.18 mL	0.90 mL	1.80 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Sulfo-NHS-LC-Biotin

References are publications that support the biological activity of the product.

Xu and Wegner Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci.
Karunakaran Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 2020 PMID: 32155411
Takayama Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 2017 PMID: 29208980