MTT
Tocris Bioscience, part of Bio-Techne | Catalog # 5224
Reagent used in the measurement of cell proliferation
Key Product Details
Description
Reagent used in the measurement of cell proliferation
Product Description
MTT is a reagent used in the measurement of cell proliferation.Product Specifications
Molecular Weight
414.32
Formula
C18H16N5SBr
Storage
Store at +4°C
Purity
≥98%
Chemical Name
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
CAS Number
298-93-1
PubChem ID
64965
InChI Key
AZKSAVLVSZKNRD-UHFFFAOYSA-M
SMILES
CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| PBS | 5 |
Preparing Stock Solutions
for MTT
The following data is based on the product molecular weight 414.32
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.13 mM | 18.57 mL | 92.83 mL | 185.66 mL |
| 0.65 mM | 3.71 mL | 18.57 mL | 37.13 mL |
| 1.3 mM | 1.86 mL | 9.28 mL | 18.57 mL |
| 6.5 mM | 0.37 mL | 1.86 mL | 3.71 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for MTT
For research use only
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