IA-Alkyne

Product Description

IA-Alkyne is a broad spectrum cysteine reactive probe. Covalently binds cysteine residues in proteins. Can be utilized for covalent fragment-based ligand discovery (FBLD) and to identify Zn²⁺-binding cysteines. Used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. Covalently binds cysteine residues in and activates TRPA1 channels.

Solubility

Preparing Stock Solutions for IA-Alkyne

The following data is based on the product molecular weight 265.09

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.77 mL	18.86 mL	37.72 mL
5 mM	0.75 mL	3.77 mL	7.54 mL
10 mM	0.38 mL	1.89 mL	3.77 mL
50 mM	0.08 mL	0.38 mL	0.75 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Pace and Weerapana A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem.Biol. 2014 PMID: 24111988
• Macpherson Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 2007 PMID: 17237762
• Backus Proteome-wide covalent ligand discovery in native biological systems. Nature 2016 PMID: 27309814