I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?

Name: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)
Brand: Novus Biologicals
SKU: NBP1-71713

As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.

Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71713

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Product Summary
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
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Key Product Details

Applications

Chromatin Immunoprecipitation

Kit Type

Kit (ChIP)

Product Summary for Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

This ChIP kit is specific for Histone H3 monomethyl Lysine 9, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS). This kit comes with a full size vial of Histone H3 Monomethyl Lys9 Antibody (NB21-1071).

Scientific Data Images for Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit [NBP1-71713] - 2 ug of NB21-1023 was used to IP DNA from fixed HeLa cells along side a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit [NBP1-71713] - Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.

Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.

Kit Contents for Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

10X Glycine (30 mL)
DNA Binding Buffer (18 mL)
DNA Elution Buffer (2 mL)
DNA Wash Buffer (5 mL - add 20 mL EtOH)
H3 K4Me3 Positive Control Antibody (0.025 mg) - NB21-1023SS
H3 Monomethyl Lys9 Antibody (0.05 mg) - NB21-1071
Human AFM Negative Control Primer Pair at 10 uM (0.5 mL) - NBP171653
Human RPL30 Positive Control Primer Pair at 10 uM (0.5 mL) - NBP1-71651
IP Dilution Buffer (2 x 25 mL)
IP Elution Buffer (7 mL)
IP Wash Buffer 1 (15 mL)
IP Wash Buffer 2 (15 mL)
IP Wash Buffer 3 (15 mL)
IP Wash Buffer 4 (15 mL)
Protein A/G Magnetic Beads (0.8 mL)
Proteinase K (0.06 mL)
Purification Columns (30 EA)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: Chromata ChIP Histone H3

Novus' ChromataChIP Kits are a complete pack to help you perform Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer. ChromataChIP Kits come with everything you need to perform Chromatin IP including a colorful, step-by-step protocol pamphlet to walk you through the 4 main phases of ChIP. Learn more by visiting www.novusbio.com/ChromataChIP

Alternate Names

H3K9Me1

Additional Chromata ChIP Histone H3 Products

Product Documents for Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ChromataChIP Kit Protocol NBP1-71713

COA

Product Specific Notices for Chromata ChIP Histone H3 [Monomethyl Lys9] Kit (ChIP)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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