Cell Activation Cocktail 500X

Name: Cell Activation Cocktail 500X
Brand: Tocris Bioscience
SKU: 5476
Rating: 5 (0 reviews)

Catalog # 5476 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Cell activation cocktail

Key Product Details

Description:

Cell activation cocktail

View Full Technical Details for Cell Activation Cocktail 500X

Biological Activity for Cell Activation Cocktail 500X

Cell Activation Cocktail 500X contains the following compounds: Monensin sodium salt (1.5 mM), Phorbol 12-myristate 13-acetate (0.0405 mM) and Ionomycin calcium salt (0.67 mM). Cocktail is provided as 1 ml of 500X concentrate in ethanol.

Technical Data for Cell Activation Cocktail 500X

Storage	Store at -80°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cell Activation Cocktail 500X

Solubility

Soluble in ethanol (supplied pre-dissolved in 1ml ethanol)

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Customer Reviews for Cell Activation Cocktail 500X (2)

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Cell Activation Cocktail

Name: Anonymous

Assay Type: In Vitro

Cell Line/Tissue: splenocytes/ T cells

This product was used to perform a TH17 differentiation assay using a flow cytometry analysis afterwards. This cocktail has made cell activation easier and my experimental preparation move along much faster.
Used to stimulate tumor infiltrating lymphocytes after isolation

Name: Stephanie Watkins

Assay Type: In Vitro

Cell Line/Tissue: T cells

Tumor infiltrating lymphocytes (TILs) were isolated from mouse liver tumors and then stimulated with 1X cell activation cocktail (diluted in RPMI media). After 5 hour activation, cytokine production on the TILs was measured via flow cytometry. Example picture shows CD107A expression before and after activation.

Always use it at 1X.

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Product Documents for Cell Activation Cocktail 500X

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.