Auxin

Name: Auxin
Brand: Tocris Bioscience
SKU: 6834

Tocris Bioscience, part of Bio-Techne | Catalog # 6834

Citations (4)

COA / SDS

Chemical dimerizer used in auxin-inducible degron (AID) system; phytohormone

Key Product Details

Description:	Chemical dimerizer used in auxin-inducible degron (AID) system; phytohormone
Chemical Name:	1H-Indole-3-acetic acid
Purity:	≥98% (HPLC)
Molecular Weight:	175.18

View Full Technical Details for Auxin

Technical Data
Citations

Biological Activity for Auxin

Auxin is a chemical dimerizer used in auxin-inducible degron (AID) systems. Induces degradation of a target protein tagged with the auxin-receptor F-box protein Tir1 E3 ligase AID in human colorectral cancer and mouse ES cells or tagged with AFB2 in A431 cells. Endogenous plant hormone.

Technical Data for Auxin

M. Wt	175.18
Formula	C₁₀H₉NO₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	87-51-4
PubChem ID	802
InChi Key	SEOVTRFCIGRIMH-UHFFFAOYSA-N
Smiles	OC(CC1=CNC2=C1C=CC=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Auxin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	17.52	100
	ethanol	17.52	100

Preparing Stock Solutions for Auxin

The following data is based on the product molecular weight 175.18.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.71 mL	28.54 mL	57.08 mL
5 mM	1.14 mL	5.71 mL	11.42 mL
10 mM	0.57 mL	2.85 mL	5.71 mL
50 mM	0.11 mL	0.57 mL	1.14 mL

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Reconstitution Calculator

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References for Auxin

References are publications that support the biological activity of the product.

Natsume Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 2016 PMID: 27052166
Li An efficient auxin-inducible degron system with low basal degradation in human cells. Nat.Methods. 2019 PMID: 31451765
Nishimura An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat.Methods 2009 PMID: 19915560