AbK
Tocris Bioscience, part of Bio-Techne | Catalog # 5113
Photo-crosslinking probe; unnatural amino acid
Key Product Details
Description
Photo-crosslinking probe; unnatural amino acid
Product Description
AbK is an unnatural amino acid. Acts as a UV light-activated (369 nm) photocrosslinking probe when incorporated into proteins by pyrrolysyl tRNA synthetase/tRNACUA pairs.Product Specifications
Molecular Weight
272.30
Formula
C11H20N4O4.CF3CO2H
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N6-[[2-(3-Methyl-3H-diazirin-3-yl)ethoxy]carbonyl]-L-lysine
CAS Number
1253643-88-7
PubChem ID
66825403
InChI Key
LUCMNTLJAFTFDU-QMMMGPOBSA-N
SMILES
N[C@H](C(O)=O)CCCCNC(OCCC1(C)N=N1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 2.72 | 10 with gentle warming | |
| 1eq. HCl | 27.23 | 100 |
Preparing Stock Solutions
for AbK
The following data is based on the product molecular weight 272.30
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.67 mL | 18.36 mL | 36.72 mL |
| 5 mM | 0.73 mL | 3.67 mL | 7.34 mL |
| 10 mM | 0.37 mL | 1.84 mL | 3.67 mL |
| 50 mM | 0.07 mL | 0.37 mL | 0.73 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Kleiner A chemical proteomics approach to reveal direct protein-protein interactions in living cells. Cell Chem.Biol. 2018 PMID: 29104064
- Chatterjee Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc.Natl.Acad.Sci.U.S.A. 2013 PMID: 23818609
- Lowder Visualizing protein partnerships in living cells and organisms. Curr.Opin.Cell Biol. 2011 PMID: 22104179
- Ai Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem 2011 PMID: 21678540
Product Specific Notices for AbK
For research use only
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