StemXVivo Ectoderm Kit
R&D Systems, part of Bio-Techne | Catalog # SC031B
For ectoderm differentiation of human pluripotent stem cells
Key Product Details
Species
Human
Product Summary for StemXVivo Ectoderm Kit
Kit Summary
For the differentiation of human pluripotent stem cells into ectoderm.
Key Benefits
- Ectoderm differentiation in 4 days
- Optimized components to reduce experimental variation
- Contains differentiation supplements and an antibody to verify ectoderm differentiation
- Involves validated and straightforward protocols
Why Differentiate Human Pluripotent Stem Cells into Ectoderm In Vitro?
Ectoderm is one of three primary germ layers formed during the process of gastrulation in the vertebrate embryo and gives rise to the nervous system and epidermal tissues.
Ectoderm differentiation is also an essential first step for the formation of downstream derivative cell types for use in regenerative medicine, such as neurons and keratinocytes.
Ectoderm differentiation in vitro:
- Uses fully defined supplements to drive reproducible differentiation.
- Produces a healthy ectodermal cell population to increase consistency between studies and reduce unwanted experimental variability.
- Provides results in 4 days to save time and reagents.
Kit Contents
This kit contains the following reagents to drive pluripotent stem cell differentiation into ectodermal cells and an antibody to verify differentiation status:
- Ectoderm Base Media Supplement (50X)
- Ectoderm Differentiation Supplement
- Ectoderm Marker: Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody
The quantity of each component in this kit is sufficient to make 150 mL of media for differentiation. This is enough media for the differentiation of six 60 mm plates or two 24-well plates.
Scientific Data Images for StemXVivo Ectoderm Kit
Differentiation of Pluripotent Stem Cells into Ectoderm.
BG01V human embryonic stem cells were differentiated into ectoderm using the media supplements included in this kit. To evaluate lineage commitment, the cells were stained with the Anti-Human Otx2 antibody included. The cells were stained using NorthernLights™(NL)557-conjugated Donkey anti-Goat IgG secondary antibody (R&D Systems, Catalog # NL001; red), and the nuclei were counterstained with DAPI (blue).Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Pluripotent Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.
R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.
Additional Pluripotent Stem Cells Products
Product Documents for StemXVivo Ectoderm Kit
Product Specific Notices for StemXVivo Ectoderm Kit
For research use only
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