Human Pluripotent Stem Cell Marker Antibody Panel Plus

Kit Summary

Eight antibodies to verify human stem cell pluripotency.

Why Use Molecular Markers to Verify Human Stem Cell Pluripotency?

Stem cell pluripotency can be characterized functionally by their ability to differentiate into cells of the three vertebrate germ layers. However, functional verification is time-consuming, expensive, and is less conducive to routine stem cell culture screening or high throughput analysis of stem cell pluripotency.

In addition to the ability to give rise to all cell types, a number of molecular markers have been identified to characterize the pluripotent status of stem cells. For example, human pluripotent stem cells express the cell surface proteins SSEA-4 and Alkaline Phosphatase and the transcription factors Oct-3/4 and Nanog.

Analyzing human stem pluripotency using marker antibodies:

• Promotes the identification and expansion of high quality, undifferentiated stem cell populations.
• Provides increased confidence in pluripotent status through the use of multiple markers.
• Defines the starting stem cell population to reduce experimental variation.
• Is a straightforward, cost-effective way to verify stem cell pluripotency.

Kit Contents

This kit contains the following antibodies to verify human stem cell pluripotency:

Positive Markers

• Mouse Anti-Human CD9 Monoclonal Antibody
• Mouse Anti-Human E-Cadherin Monoclonal Antibody
• Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody
• Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody
• Mouse Anti-Human PODXL (GCTM antigen) Monoclonal Antibody
• Mouse Anti-Human SSEA-4 Monoclonal Antibody
• Mouse Anti-Human SOX2 Monoclonal Antibody

Negative Marker

• Mouse Anti-Human SSEA-1 Monoclonal Antibody

Each antibody is supplied as 25 µg; if reconstituted in 250 µL, this provides reagents sufficient for processing eight 300 µL immunocytochemistry samples or 25 flow cytometry samples.