Real Blue Peroxidase Substrate Kit

Novus Biologicals, part of Bio-Techne | Catalog # KA0565

Novus Biologicals, part of Bio-Techne
Discontinued Product
KA0565 has been discontinued. View all Real Blue Peroxidase products.

Key Product Details

Species

Mouse

Applications

Immunohistochemistry

Kit Type

Substrate Kit

Product Summary for Real Blue Peroxidase Substrate Kit

Quality control test: IHC demonstration data

Materials Required but Not Supplied: Primary antibody; HRP labeled reagent; Isopropyl alcohol; Serum Block: 10% normal serum from the species the secondary antibody was made in; DAB for double staining; Organic Mounting Media; 0.1 M Tris HCl or PBS (See Reagent Preparation). RealBlue Peroxidase Substrate Kit is 50-100 times more sensitive than DAB and is non-carcinogenic. Strongly recommended for the detection of least-abundant markers. RealBlue forms a blue chromogenic product, providing excellent contrast with DAB and other substrates for multiple labeling experiments.

Product Specifications

Applications

Immunohistochemistry (1:10-1:500)
Immunohistochemistry-Paraffin (1:10-1:500)

Scientific Data Images for Real Blue Peroxidase Substrate Kit

Immunohistochemistry-Paraffin: Real Blue Peroxidase Substrate Kit [KA0565]

Immunohistochemistry-Paraffin: Real Blue Peroxidase Substrate Kit [KA0565]

Immunohistochemistry-Paraffin: Real Blue Peroxidase Assay Kit [KA0565] - IHC-P staining of epithelial membrane antigen in kidney tissue using chromogen reagent Real Blue Peroxidase Assay Kit (KA0565)

Kit Contents for Real Blue Peroxidase Substrate Kit

  • Blocking Solution Concentrate (10X) (10 mL)
  • Orcein (50 mL)
  • RealBlue Peroxidase Substrate (100 mL)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at room temperature.

Background: Real Blue Peroxidase

The application of antibodies and other proteins covalently coupled to horseradish peroxidase (HRP) in immunohistochemistry is well documented. In the presence of hydrogen peroxide (H2O2), HRP catalyzes the oxidation of TMB forming a blue chromogenic product visible by light microscopy. Researchers have shown that the reaction of TMB with HRP is a sensitive method for demonstration of retrograde tracing in neural tissue. Lu and Ho obtained excellent staining with TMB-molybdate in anterograde labeling of neural tissue at the ultrastructure level. When used in double-labeling for localization of innervations of feline cerebral arteries, TMB and DAB peroxidase substrates provided high resolution morphological results. Previous methods to stabilize the blue reaction color required multiple staining steps, but RealBlue provides permanent blue color when used as recommended. Like DAB, the RealBlue reaction product is insoluble in alcohols and Xylene. Endogenous peroxidase activity is eliminated by controlled oxidation prior to application of antibodies

Product Documents for Real Blue Peroxidase Substrate Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Real Blue Peroxidase Substrate Kit

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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