NEUROBIOTIN(R) [351 Tracer]
Novus Biologicals, part of Bio-Techne | Catalog # SP-1155-2-NB
Discontinued Product
SP-1155-2-NB has been discontinued.
View all NEUROBIOTIN(R) products.
Key Product Details
Conjugate
351 Tracer
Product Summary for NEUROBIOTIN(R) [351 Tracer]
NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.
FeaturesBright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
Biotin label with a biotinidase-resistant linkage
Fixable primary amine
Used for visualizing neural architecture and for the identification of gap junction coupling
Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labelsBetter solubility
More efficiently iontophoresed
Remains in cell longer
Non-toxic
Can be fixed with formalin or glutaraldehyde
Features
Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels
Product Specifications
Application Notes
Once in solution, store frozen. Usage NEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: a biotin label with a biotinidase-resistant linker, a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor 350, a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde..
Scientific Data Images for NEUROBIOTIN(R) [351 Tracer]
NEUROBIOTIN(R) [351 Tracer] [SP-1155-2-NB]
Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Desiccate at +4C
Product Documents for NEUROBIOTIN(R) [351 Tracer]
Product Specific Notices for NEUROBIOTIN(R) [351 Tracer]
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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