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M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit

Novus Biologicals, part of Bio-Techne | Catalog # BMK-2202-NB

Novus Biologicals, part of Bio-Techne
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BMK-2202-NB

Key Product Details

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Kit Type

Immunodetection Kit

Product Summary for M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit contains a proprietary Mouse IgG Blocking Reagent and an M.O.M.(R). Biotinylated Anti-Mouse IgG reagent and is Based on the simple and easy to follow avidin or streptavidin/biotin reagent protocols. An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection, or choose a fluorochrome-labeled avidin or streptavidin.
The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. The M.O.M.(R) Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems.

Product Specifications

Application Notes

The M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections..

Kit Contents for M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit

  • 0.1 ml M.O.M.(R) (Mouse-On-Mouse) Biotinylated Anti-Mouse IgG Reagent
  • 1 ml Mouse IgG Blocking Reagent
  • 6 ml of M.O.M.(R) (Mouse-On-Mouse) Protein Concentrate

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: M.O.M.(R) (Mouse-On-Mouse) Basic

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit. The M.O.M.(R) (Mouse-On-Mouse) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.

Additional M.O.M.(R) (Mouse-On-Mouse) Basic Products

Product Documents for M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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