ImmPACT (TM) DAB EqV HRP Substrate

Name: ImmPACT (TM) DAB EqV HRP Substrate
Brand: Novus Biologicals
SKU: SK-4103-NB

Novus Biologicals, part of Bio-Techne | Catalog # SK-4103-NB

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Product Summary
Product Specifications
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Applications

ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Product Summary for ImmPACT (TM) DAB EqV HRP Substrate

Features:
Optimized for IHC/ICC/ISH Suitable for single and multiple labeling Heat stable Permanent mounting Ideal for single and multiple labeling Can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes Working solution stable for several days at 4C One year expiry date Consists of 200 mL each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution

Product Specifications

Application Notes

ImmPACT(TM) DAB EqV (equal volume) consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution. ImmPACT(TM) DAB EqV produces a dark brown reaction product that is 3 to 5 times more sensitive than the original DAB substrate. The working solution is stable for several days when stored at 4C. This substrate can be used for both manual and automated staining methods. ImmPACT(TM) DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted..

Kit Contents for ImmPACT (TM) DAB EqV HRP Substrate

200 ml ImmPACT™ DAB EqV Reagent 1
200 ml ImmPACT™ DAB EqV Reagent 2

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: ImmPACT (TM) DAB EqV HRP Substrate

DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.

Additional ImmPACT (TM) DAB EqV HRP Substrate Products

Product Documents for ImmPACT (TM) DAB EqV HRP Substrate

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ImmPACT DAB EqV HRP Substrate Protocol

COA

SDS

Download SDS

Product Specific Notices for ImmPACT (TM) DAB EqV HRP Substrate

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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