Withaferin A
Tocris Bioscience, part of Bio-Techne | Catalog # 2816
Inhibits NF-κB activation
Discontinued Product
2816 has been discontinued.
View all NFkB/IkB Inhibitors products.
Product Description
Withaferin A is a steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Withaferin A inhibits endothelial cells (HUVEC) sprouting in vitro (IC50 = 12 nM) and in vivo, prevents NF-κB activation by inhibiting activation of IKKβ and inhibits chymotrypsin-like activity of the 20S proteasome. In an animal model of Huntington's disease (HD), Withaferin A delays disease progression and increases lifespan; in a cellular model it suppresses aggregation of mutant huntingtin, activates HSF1 and induces Hsp70 chaperone expression.Product Specifications
Molecular Weight
470.60
Formula
C28H38O6
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
CAS Number
5119-48-2
PubChem ID
45489105
InChI Key
DBRXOUCRJQVYJQ-WDBPBROTSA-N
SMILES
O=C1[C@@]2(C)[C@@]([C@H]5C[C@]3([H])C2CC[C@@]4(C)[C@]([H])3CC[C@@H]4[C@@H]([C@]6([H])OC(C(CO)=C(C)C6)=O)C)(O5)[C@@H](O)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.71 | 10 |
Preparing Stock Solutions
for Withaferin A
The following data is based on the product molecular weight 470.60
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 21.25 mL | 106.25 mL | 212.49 mL |
| 0.5 mM | 4.25 mL | 21.25 mL | 42.50 mL |
| 1 mM | 2.12 mL | 10.62 mL | 21.25 mL |
| 5 mM | 0.42 mL | 2.12 mL | 4.25 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Joshi Withaferin A induces heat shock response and ameliorates disease progression in a mouse model of Huntington's disease. Mol.Neurobiol. 2021 PMID: 33904021
- Yang The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry". Mol.Pharmacol. 2007 PMID: 17093135
- Kaileh Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity. J.Biol.Chem. 2007 PMID: 17150968
- Mohan Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 2004 PMID: 15516832
Product Specific Notices for Withaferin A
For research use only
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