Wiskostatin

Name: Wiskostatin
Brand: Tocris Bioscience
SKU: 4434

Tocris Bioscience, part of Bio-Techne | Catalog # 4434

Citations (16)

COA / SDS

N-WASP inhibitor; inhibits Arp2/3 activation

Key Product Details

Description:	N-WASP inhibitor; inhibits Arp2/3 activation
Chemical Name:	3,6-Dibromo-α-[(dimethylamino)methyl]-9H-cabazole-9-ethanol
Purity:	≥98% (HPLC)
Molecular Weight:	426.15

View Full Technical Details for Wiskostatin

Technical Data
Citations

Biological Activity for Wiskostatin

Wiskostatin is an inhibitor of neural Wiskott-Aldrich syndrome protein (N-WASP) activity. Interacts with the regulatory GTPase-binding domain of N-WASP; inhibits activation of Arp2/3 complex by maintaining N-WASP in an inactive conformation. Also inhibits PIP₂-induced actin polymerization (EC₅₀ ~ 4μM).

Compound Libraries for Wiskostatin

Wiskostatin is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Wiskostatin

M. Wt	426.15
Formula	C₁₇H₁₈Br₂N₂O
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	253449-04-6
PubChem ID	2775510
InChi Key	XUBJEDZHBUPBKL-UHFFFAOYSA-N
Smiles	BrC(C=C3)=CC1=C3N(CC(O)CN(C)C)C2=C1C=C(Br)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Wiskostatin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.62	100

Preparing Stock Solutions for Wiskostatin

The following data is based on the product molecular weight 426.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.35 mL	11.73 mL	23.47 mL
5 mM	0.47 mL	2.35 mL	4.69 mL
10 mM	0.23 mL	1.17 mL	2.35 mL
50 mM	0.05 mL	0.23 mL	0.47 mL

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References for Wiskostatin

References are publications that support the biological activity of the product.

Peterson Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat.Struct.Mol.Biol. 2004 PMID: 15235593
Guerriero and Weisz N-WASP inhibitor wiskostatin nonselectively perturbs membrane transport by decreasing cellular ATP levels. Am.J.Physiol.Cell Physiol. 2006 PMID: 17092993
Wegner N-WASP and the Arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. J.Biol.Chem. 2008 PMID: 18430734