TPEN

Name: TPEN
Brand: Tocris Bioscience
SKU: 4309

Tocris Bioscience, part of Bio-Techne | Catalog # 4309

Citations (13)

COA / SDS

Cell-permeable Zn2+ chelator; also inhibits RNA binding protein Lin28

Key Product Details

Description:	Cell-permeable Zn²⁺ chelator; also inhibits RNA binding protein Lin28
Chemical Name:	N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine
Purity:	≥97% (HPLC)
Molecular Weight:	424.54

View Full Technical Details for TPEN

Technical Data
Citations

Biological Activity for TPEN

TPEN is a heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK₁ cells; acts as an intracellular chelator of proteomic Zn²⁺. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Also inhibits RNA binding protein Lin28 (IC₅₀ = 2.5 μM). Cell permeable.

Technical Data for TPEN

M. Wt	424.54
Formula	C₂₆H₂₈N₆
Storage	Store at +4°C
Purity	≥97% (HPLC)
CAS Number	16858-02-9
PubChem ID	5519
InChi Key	CVRXLMUYFMERMJ-UHFFFAOYSA-N
Smiles	N(CC3=CC=CC=N3)(CC4=CC=CC=N4)CCN(CC2=CC=CC=N2)CC1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TPEN

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	10.61	25
	ethanol	42.45	100

Preparing Stock Solutions for TPEN

The following data is based on the product molecular weight 424.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.36 mL	11.78 mL	23.55 mL
5 mM	0.47 mL	2.36 mL	4.71 mL
10 mM	0.24 mL	1.18 mL	2.36 mL
50 mM	0.05 mL	0.24 mL	0.47 mL

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References for TPEN

References are publications that support the biological activity of the product.

Wang Small-molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28. Cell Rep. 2018 PMID: 29874593
Hyun Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells. Invest.Opthalmol.Vis.Sci.
Rana Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. J.Inorg.Biochem. 2008 PMID: 18171589
Matias Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2. J.Fluoresc. 2010 PMID: 19821015
Chimienti Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors. Biochem.Pharmacol. 2001 PMID: 11377396