T3

Name: T3
Brand: Tocris Bioscience
SKU: 6666

Tocris Bioscience, part of Bio-Techne | Catalog # 6666

Citations (4)

COA / SDS

Thyroid hormone; also promotes differentiation of oligodendroglial precursor cells

Key Product Details

Description:	Thyroid hormone; also promotes differentiation of oligodendroglial precursor cells
Chemical Name:	O-(4-Hydroxy-3-iodophenyl-3,5-diiodo-L-tyrosine
Purity:	≥98% (HPLC)
Molecular Weight:	650.97

View Full Technical Details for T3

Technical Data
Citations

Biological Activity for T3

T3 is a thyroid hormone. Promotes differentiation of oligodendroglial precursor cells in neonatal rats and promotes maturation of hiPSC-derived cardiomyocytes. Also specifies cone cell subtype in retinal cell differentiation protocols. T3 is used to generate cortical spheroids and β cells (see protocol below) from hPSCs.

T3 synthesized to Ancillary Material Grade also available.

For more information about how T3 may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for T3

M. Wt	650.97
Formula	C₁₅H₁₂I₃NO₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	6893-02-3
PubChem ID	5920
InChi Key	AUYYCJSJGJYCDS-LBPRGKRZSA-N
Smiles	N[C@H](C(O)=O)CC1=CC(I)=C(C(I)=C1)OC2=CC(I)=C(C=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for T3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	32.55	50

Preparing Stock Solutions for T3

The following data is based on the product molecular weight 650.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.07 mL	15.36 mL	30.72 mL
2.5 mM	0.61 mL	3.07 mL	6.14 mL
5 mM	0.31 mL	1.54 mL	3.07 mL
25 mM	0.06 mL	0.31 mL	0.61 mL

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References for T3

References are publications that support the biological activity of the product.

Surendran An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 2022 PMID: 36386870
Eldred Thyroid hormone signaling specifies cone subtypes in human retinal organoids. Science 2018 PMID: 30309916
Madhavan Induction of myelinating oligodendrocytes in human cortical spheroids. Nat Methods. 2018 PMID: 30046099
Yang Tri-iodo-L-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells. J.Mol.CellCardiol. 2014 PMID: 24735830
Marta Sustained neonatal hyperthyroidism in the rat affects myelination in the central nervous system. J.Neurosci.Res. 1998 PMID: 9671982

T3