sCy5DL-amide
Tocris Bioscience, part of Bio-Techne | Catalog # 7835
FDAA for super-resolution microscopy of bacteria
Key Product Details
Description
FDAA for super-resolution microscopy of bacteria
Product Description
sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.Scientific Data Images for sCy5DL-amide
Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide
Optical Data
| Emission Color | Red |
| λabs | 646 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 209,200 M-1cm-1 |
| Cell Permeable | Yes |
| Application | STORM |
Product Specifications
Molecular Weight
769.97
Formula
C38H51N5O8S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate
InChI Key
KASKHZOOXRBMET-SSEXGKCCSA-N
SMILES
CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.7 | 10 |
Preparing Stock Solutions
for sCy5DL-amide
The following data is based on the product molecular weight 769.97
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 12.99 mL | 64.94 mL | 129.88 mL |
| 0.5 mM | 2.60 mL | 12.99 mL | 25.98 mL |
| 1 mM | 1.30 mL | 6.49 mL | 12.99 mL |
| 5 mM | 0.26 mL | 1.30 mL | 2.60 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhang Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 2022 PMID: 35994360
Product Specific Notices for sCy5DL-amide
For research use only
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