SANT-1

Name: SANT-1
Brand: Tocris Bioscience
SKU: 1974

Tocris Bioscience, part of Bio-Techne | Catalog # 1974

Citations (68)

COA / SDS

Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity

Key Product Details

Description:	Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Chemical Name:	N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
Purity:	≥98% (HPLC)
Molecular Weight:	373.5

View Full Technical Details for SANT-1

Technical Data
Citations

Biological Activity for SANT-1

SANT-1 is a potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (K_D = 1.2 nM). Inhibits smoothened agonist effects with an IC₅₀ of 20 nM (in Shh-LIGHT2 cells). Used in protocols to generate pancreatic β cells from hPSCs (see our protocol below).

For more information about how SANT-1 may be used, see our protocol: Generation of β cells from hPSCs

Compound Libraries for SANT-1

SANT-1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SANT-1

M. Wt	373.5
Formula	C₂₃H₂₇N₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	304909-07-7
PubChem ID	6878030
InChi Key	FOORCIAZMIWALX-JJIBRWJFSA-N
Smiles	CC1=NN(C(C)=C1\C=N\N1CCN(CC2=CC=CC=C2)CC1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SANT-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	18.68	50

Preparing Stock Solutions for SANT-1

The following data is based on the product molecular weight 373.5.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.35 mL	26.77 mL	53.55 mL
2.5 mM	1.07 mL	5.35 mL	10.71 mL
5 mM	0.54 mL	2.68 mL	5.35 mL
25 mM	0.11 mL	0.54 mL	1.07 mL

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References for SANT-1

References are publications that support the biological activity of the product.

Chen Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci.USA.