RIPA 56

Name: RIPA 56
Brand: Tocris Bioscience
SKU: 7339

Tocris Bioscience, part of Bio-Techne | Catalog # 7339

COA / SDS

Potent and metabolically stable RIP1 kinase inhibitor

Key Product Details

Description:	Potent and metabolically stable RIP1 kinase inhibitor
Chemical Name:	N-Hydroxy-2,2-dimethyl-N-(phenylmethyl)butanamide
Purity:	≥98% (HPLC)
Molecular Weight:	221.3

View Full Technical Details for RIPA 56

Technical Data
Citations

Biological Activity for RIPA 56

RIPA 56 is a potent and metabolically stable receptor-interacting protein kinase 1 (RIPK1) inhibitor (EC₅₀ = 13 nM). RIPA-56 shows no inhibition of RIP3 kinase at 10 μM. RIPA 56 blocks necroptosis and aging of the mouse male reproductive system. RIPA 56 reduces tumor necrosis factor α (TNFα)-induced mortality and multi-organ damage in a mouse model of systemic inflammatory response syndrome (SIRS). RIPA 56 is orally bioavailable.

Compound Libraries for RIPA 56

RIPA 56 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for RIPA 56

M. Wt	221.3
Formula	C₁₃H₁₉NO₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1956370-21-0
PubChem ID	121439991
InChi Key	AVYVHIKSFXVDBG-UHFFFAOYSA-N
Smiles	O=C(N(O)CC=1C=CC=CC1)C(C)(C)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RIPA 56

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	22.13	100
	ethanol	22.13	100

Preparing Stock Solutions for RIPA 56

The following data is based on the product molecular weight 221.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.52 mL	22.59 mL	45.19 mL
5 mM	0.90 mL	4.52 mL	9.04 mL
10 mM	0.45 mL	2.26 mL	4.52 mL
50 mM	0.09 mL	0.45 mL	0.90 mL

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Reconstitution Calculator

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References for RIPA 56

References are publications that support the biological activity of the product.

Ren Discovery of a highly potent, selective, and metabolically stable inhibitor of receptor-interacting protein 1 (RIP1) for the treatment of systemic inflammatory response syndrome. J.Med.Chem. 2017 PMID: 27992216
Zhuang Small-molecule inhibitors of necroptosis: current status and perspectives. J.Med.Chem. 2019 PMID: 31622096
Li RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system. Elife 2017 PMID: 28807105