Key information: RiboDyEE™ RNA Dye is an ultra-sensitive green, fluorescent dye specifically developed in a user-friendly format for RNA-LNP encapsulation efficiency (EE) assays. Rigorously validated against the leading competitor, it delivers equivalent performance, with enhanced convenience and fewer handling steps.
Application: RiboDyEE is used for the precise quantification of RNA encapsulated within lipid nanoparticles (LNPs) and can detect low RNA concentrations ranging from 1 to 200 ng.
Format: RiboDyEE provides a stable, more usable and cost-effective solution compared to the leading competitor product. Supplied in convenient 120 μL aliquots (5 aliquots per pack), each RiboDyEE aliquot is sufficient for accurately measuring up to 21 RNA samples in total, across 3 x 96-well plates (for each plate: 7 different RNA samples, in triplicate, + 1 blank and calibration curves), using a protocol specifically optimized for determining encapsulation efficiency. Additionally, each aliquot can be used to generate standard calibration curves under two conditions, with and without detergent, enhancing quantification accuracy while minimizing degradation caused by repeated freeze-thaw cycles.
Properties and Photophysical Data: The dye exhibits excitation and emission maxima (λ) at 485 nm and 525 nm, respectively, with over 1000-fold fluorescence enhancement and a high quantum yield of 0.65 upon RNA binding.
Encapsulation Efficiency Assay: For instructions and expert tips on optimizing encapsulation efficiency using RiboDyEE, please consult the provided protocol and application note. This optimized assay accounts for detergent effects, advises preparing standards from the specific RNA species being encapsulated instead of generic RNA, and delivers accurate measurements of encapsulation efficiency along with the percentage of RNA recovery.
Request our data analysis spreadsheet to help with your encapsulation efficiency data analysis.
RiboDyEE is a trademark of Bio-Techne Corp.
